General Cell Based Assays
Eurofins Discoverx PathHunter eXpress XBP1 Nuclear Translocation Assay, 2-Plate Kit. (93-0857E3CP5M)
The PathHunter eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Mouse Macrophage Migration Inh
Mouse Macrophage Migration Inhibitory Factor MIF ELISA Kit from CUSABIO Detection Range 125 pg/ml - 8000 pg/ml Sensitivity 31 25 pg/ml Sample serum plasma tissue homogenates Method Sandwich
Eurofins Discoverx PathHunter eXpress LHCGR CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0957E2CP0M)
The PathHunter LHCGR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. luteinizing hormone) based activation of LHCGR GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LHCGR and EA-tagged β-Arrestin-2. Activation of LHCGR stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical RXR aRetInoId X RECPTR a 100uL
An affinity probe for ganglioside GM3 binding partners
ABclonal Technology HRP-conjugated B-Actin
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
Eurofins Discoverx PathHunter eXpress Flt4 Activity Assay, 10-Plate Kit. (93-0888E3CP16L)
The PathHunter FLT4 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT4 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT4 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CXCR3 Activated GPCR Internalization Assay, 10pl Kit. (93-0661E3CP0L)
The PathHunter CXCR3 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CXCR3 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CXCR3. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CXCR3-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
GPCR Kinase 2 Expression Kit - BacMam, 10mL - Montana Molecular
GPCR Kinase 2 (GRK2) packaged in BacMam for expression in mammalian cells. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity, enabling rapid assay optimization. Co-express with other Montana Molecular tools includeing GPCRs packaged in BacMam or biosensors for cAMP, DAG, or arrestin signaling.
Cayman Chemical Acetaldehyde asy Reagent 200uL
A Clk1 Clk2 and Clk4 inhibitor (IC50s 8 20 and 12 nM respectively) selective for these kinases over Clk3 (IC50 >3000 nM) as well as DYRK1A DYRK1B DYRK2 HIPK1 and HIPK3 (IC50s 213 956 1309 187 and 159 nM respectively) but does inhibit HIPK2 (IC50 23 nM)
Eurofins Discoverx cAMP Hunter eXpress GPR35 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0152E2CP2L)
The cAMP Hunter GPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of GPR35 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR35, a naturally Gi/Go coupled GPCR. Upon activation of GPR35, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Amsbio LLC Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 500 Assay Points
Indigo Biosciences Human Adrenoceptor Alpha 1B (ADRA1B) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Revvity Health Sciences Inc HTRF Human and Mouse Phospho-p62/SQSTM1 (Ser403) Detection Kit, 500 Assay Points
HTRF Human and Mouse Phospho-p62/SQSTM1 (Ser403) Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress mSSTR2 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0616E2MCP2L)
The PathHunter mSSTR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. somatostatin 28) based activation of mSSTR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mSSTR2 and EA-tagged β-Arrestin-2. Activation of mSSTR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.